Invitrogen

GSDMD Polyclonal Antibody

GSDMD Antibody in Immunocytochemistry (ICC/IF)

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GSDMD Antibody (PA5-115330) in ICC/IF

Immunocytochemistry analysis of GSDMD in HeLa cells (LPS treated 4h). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115330) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

GSDMD Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

GSDMD Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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产品信息

PA5-115330

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:50-1:200

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免疫细胞化学 (ICC/IF)

1:50-1:200

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human GSDMD(Accession P57764), corresponding to amino acid residues W50-G100.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2899966

产品详细信息

Antibody detects endogenous levels of total GSDMD protein, including precursor and the cleaved N-terminal fragment.

靶标信息

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Ticagrelor reduces doxorubicin-induced pyroptosis of rat cardiomyocytes by targeting GSK-3β/caspase-1.

Frontiers in cardiovascular medicine

Wang SH,Sun MJ,Ding SY,Liu CL,Wang JM,Han SN,Lin X,Li Q

Published figure using GSDMD polyclonal antibody (Product # PA5-115330) in Western Blot

Wed Feb 01 00:00:00 UTC 2023

Ticagrelor reduces doxorubicin-induced pyroptosis of rat cardiomyocytes by targeting GSK-3β/caspase-1.

Frontiers in cardiovascular medicine

Wang SH,Sun MJ,Ding SY,Liu CL,Wang JM,Han SN,Lin X,Li Q

Published figure using GSDMD polyclonal antibody (Product # PA5-115330) in Western Blot

Wed Feb 01 00:00:00 UTC 2023

Inhibition of the glycogen synthase kinase 3β-hypoxia-inducible factor 1α pathway alleviates NLRP3-mediated pyroptosis induced by high glucose in renal tubular epithelial cells.

Experimental physiology

Wan J,Jiang Z,Liu D,Pan S,Zhou S,Liu Z

PA5-115330 was used in Immunocytochemistry, Immunohistochemistry (PFA fixed), Western Blot to demonstrate that the GSK-3β-HIF-1α signalling pathway mediates HG-stimulated pyroptosis in renal tubular epithelial cells and that down-regulation of GSK-3β inhibited HG-induced pyroptosis by inhibiting the expression of HIF-1α.