Invitrogen

GSPT1/2 Polyclonal Antibody

GSPT1/2 Antibody in Immunocytochemistry (ICC/IF)

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GSPT1/2 Antibody (PA5-143976) in ICC/IF

Immunocytochemistry analysis of GSPT1/2 in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in GSPT1/2 Polyclonal antibody (Product # PA5-143976) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropria... View More

GSPT1/2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GSPT1/2 Antibody in Flow Cytometry (Flow)

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产品信息

PA5-143976

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human GSPT1,2 recombinant protein (Position: K138-D628).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3075190

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

靶标信息

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A/B is an enzyme that in humans is encoded by the GSPT1,2 gene. This gene encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ERF3A; ERF3B; Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor GTP-binding subunit ERF3B; Eukaryotic peptide chain release factor subunit 3a; Eukaryotic peptide chain release factor subunit 3b; eukaryotic polypeptide chain release factor 3; G1 to phase transition 1; G1 to S phase transition protein 1; G1 to S phase transition protein 1 homolog; G1 to S phase transition protein 2 homolog; G1-to-S phase transition 1; G1-to-S transition GTP binding protein; guanine nucleotide regulatory protein

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基因别名: 551G9.2; AI314175; AI326449; AV307676; C79774; ERF3A; ERF3B; ETF3A; G1st; GSPT1; GSPT2; Gst-1; GST1; GST2; RGD1563213