Invitrogen

GSPT2 Polyclonal Antibody

GSPT2 Antibody in Immunocytochemistry (ICC/IF)

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GSPT2 Antibody (PA5-60824) in ICC/IF

Immunofluorecent analysis of GSPT2 in human cell line PC-3 using GSPT2 Polyclonal Antibody (Product # PA5-60824). Staining shows localization to cytosol.

GSPT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GSPT2 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of GSPT2 was observed specifically in PC-3 cells and in MCF-7 cells using anti-GSPT2 Polyclonal Antibody (Product # PA5-60824) in western blot. The relative expression levels of GSPT2 within each cell line is shown using RNA-Seq. {RE}

产品信息

PA5-60824

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化（石蜡） (IHC (P))

1:20-1:50

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human GSPT2. Recombinant protein control fragment (Product #RP-100487).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2642245

产品详细信息

Immunogen sequence: TQPPTLPAGS GSNDETCTGA GYPQGKRMGR GAPVEPSREE PLVSLEGSNS AVT

Highest antigen sequence identity to the following orthologs: Mouse - 63%, Rat - 64%.

靶标信息

ERF3B encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Effect of small molecule eRF3 degraders on premature termination codon readthrough.

Nucleic acids research

Baradaran-Heravi A,Balgi AD,Hosseini-Farahabadi S,Choi K,Has C,Roberge M

PA5-60824 was used in Simple Western - Size to show that PTC readthrough by aminoglycoside G418 is considerably enhanced by eRF3a and eRF3b siRNAs and cereblon E3 ligase modulators CC-885 and CC-90009, which induce proteasomal degradation of eRF3a and eRF3b.

Mon Apr 19 00:00:00 UTC 2021

Effect of small molecule eRF3 degraders on premature termination codon readthrough.

Nucleic acids research

Baradaran-Heravi A,Balgi AD,Hosseini-Farahabadi S,Choi K,Has C,Roberge M

Mon Apr 19 00:00:00 UTC 2021

生物信息学

蛋白别名: Eukaryotic peptide chain release factor GTP-binding subunit ERF3B; Eukaryotic peptide chain release factor subunit 3b; FLJ10441; G1 to S phase transition protein 2 homolog

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基因别名: ERF3B; GSPT2; GST2

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UniProt ID: (Human) Q8IYD1

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Entrez Gene ID: (Human) 23708

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功能

translation release factor activity GTPase activity protein binding GTP binding poly(A) RNA binding translation factor

参与通路

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay cytoplasmic translational termination translational termination cell cycle