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GSTP1 Antibody (MA5-15309) in ICC/IF
Immunofluorescence analysis of Glutathione S-transferase P was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GSTP1 Monoclonal Antibody (3F2C2) (Product # MA5-15309) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More
Product Details
MA5-15309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3F2C2
Immunogen
Purified recombinant fragment of human GSTP1 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. not determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10985031
Product Specific Information
MA5-15309 targets GSTP1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15309 immunogen is purified recombinant fragment of human GSTP1 expressed in E. Coli.
MA5-15309 detects GSTP1 which has a predicted molecular weight of approximately 23kDa.
Target Information
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; Glutathione S-transferase P; GST class-pi; GSTP1-1; HGNC:4638
Gene Aliases: DFN7; FAEES3; GST3; GSTP; GSTP1; HEL-S-22; PI
UniProt ID: (Human) P09211
Entrez Gene ID: (Human) 2950
response to reactive oxygen species
negative regulation of acute inflammatory response
negative regulation of protein kinase activity
glutathione metabolic process
xenobiotic metabolic process
central nervous system development
negative regulation of biosynthetic process
negative regulation of tumor necrosis factor-mediated signaling pathway
oligodendrocyte development
organ regeneration
response to estradiol
negative regulation of interleukin-1 beta production
negative regulation of tumor necrosis factor production
cellular response to insulin stimulus
regulation of stress-activated MAPK cascade
negative regulation of stress-activated MAPK cascade
positive regulation of superoxide anion generation
response to L-ascorbic acid
common myeloid progenitor cell proliferation
nitric oxide storage
negative regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
response to amino acid
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
negative regulation of JUN kinase activity
linoleic acid metabolic process
response to ethanol
response to mercury ion
negative regulation of fibroblast proliferation
negative regulation of nitric-oxide synthase biosynthetic process
regulation of ERK1 and ERK2 cascade
negative regulation of ERK1 and ERK2 cascade
negative regulation of leukocyte proliferation
cellular response to lipopolysaccharide
cellular response to epidermal growth factor stimulus
cellular response to glucocorticoid stimulus
cellular response to cell-matrix adhesion
negative regulation of monocyte chemotactic protein-1 production
cellular oxidant detoxification
glutathione derivative biosynthetic process
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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