Invitrogen

GSTP1 Monoclonal Antibody (3F2C2)

GSTP1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

GSTP1 Antibody (MA5-15309) in ICC/IF

Immunofluorescence analysis of Glutathione S-transferase P was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GSTP1 Monoclonal Antibody (3F2C2) (Product # MA5-15309) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa F... View More

GSTP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GSTP1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Glutathione S-transferase P was observed in PC-3, HeLa, MOLT-4 and HT-29 which are reported to be positive and not in LNCaP and Hep G2 which are negative cell lines using Anti-GSTP1 Monoclonal Antibody (3F2C2) (Product # MA5-15309) in Western Blot. {RE}

产品信息

MA5-15309

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:200-1:1,000

流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3F2C2

抗原

Purified recombinant fragment of human GSTP1 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. not determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_10985031

产品详细信息

MA5-15309 targets GSTP1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15309 immunogen is purified recombinant fragment of human GSTP1 expressed in E. Coli.

MA5-15309 detects GSTP1 which has a predicted molecular weight of approximately 23kDa.

靶标信息

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; Glutathione S-transferase P; GST class-pi; GSTP1-1; HGNC:4638

查看更多收起

基因别名: DFN7; FAEES3; GST3; GSTP; GSTP1; HEL-S-22; PI

查看更多收起

UniProt ID: (Human) P09211

查看更多收起

Entrez Gene ID: (Human) 2950

查看更多收起

功能