NeoBiotechnologies

GTF2B/ TFIIB (Transcription Factor 2B) Monoclonal Antibody (PCRP-GTF2B-1D1)

GTF2B/ TFIIB (Transcription Factor 2B) Antibody in Flow Cytometry (Flow)

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GTF2B/ TFIIB (Transcription Factor 2B) Antibody (2959-MSM1-P1ABX) in Flow

Flow cytometric analysis of PFA-fixed HeLa cells. GTF2BMouse Monoclonal Antibody (PCRP-GTF2B-1D1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

GTF2B/ TFIIB (Transcription Factor 2B) Antibody in Peptide array (ARRAY)

GTF2B/ TFIIB (Transcription Factor 2B) Antibody in SDS-PAGE (SDS-PAGE)

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产品信息

2959-MSM1-P1ABX

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1-2 µg/10^6 cells

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

1-2 g/500 µg lysate

肽阵列 (Array)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

PCRP-GTF2B-1D1

抗原

Recombinant full-length human GTF2Bprotein

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。