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GTF2F2 Antibody (PA5-112334) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GTF2F2 in U251MG cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with GTF2F2 Polyclonal Antibody (Product # PA5-112334) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
产品信息
PA5-112334
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived Human Gtf2f2 fragment
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography, Protein A
保存液
PBS, pH 7
内含物
0.03% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2867070
靶标信息
GTF2F2 is required for both basal and HIV-1 Tat-activated transcription. GTF2F2 synergizes with HIV-1 Tat and the cellular co-activator Tat-SF1 during Tat-mediated transactivation of the HIV-1 LTR promoter.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; General transcription factor IIF subunit 2; general transcription factor IIF, polypeptide 2, 30kDa; TFIIF beta; TFIIF-beta; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30
基因别名: BTF4; GTF2F2; RAP30; TF2F2; TFIIF
UniProt ID: (Human) P13984
Entrez Gene ID: (Human) 2963
mRNA splicing, via spliceosome
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
fibroblast growth factor receptor signaling pathway
gene expression
positive regulation of transcription elongation from RNA polymerase II promoter
snRNA transcription from RNA polymerase II promoter
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of viral transcription
positive regulation of transcription initiation from RNA polymerase II promoter
