Invitrogen

Galectin 9 Monoclonal Antibody (RG9-35), APC, eBioscience™

Galectin 9 Antibody in Flow Cytometry (Flow)

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Galectin 9 Antibody (17-9211-82) in Flow

Mouse bone marrow cells were surface stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol, with 0.03 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-82) (left) or 0.03 µg of Galectin 9 Monoclonal Antibody, APC (Product # 17-9211-82) (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-9211-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

RG9-35

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2735039

产品详细信息

Description: This RG9-35 monoclonal antibody recognizes mouse Galectin 9, a member of the S-type lectin family. Galectin 9 is widely expressed within a majority of hematopoietic cells with the particularly high expression on CD45R(B220)-intermediate pre-B cells.

Applications Reported: This RG9-35 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RG9-35 antibody has been tested by flow cytometric analysis of mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

靶标信息

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。