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Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
GMNN inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Geminin; Geminin GMNN; OTTHUMP00000039393; RP3 369A17.3
基因别名: Gem; GMNN; MGORS6
UniProt ID: (Human) O75496
Entrez Gene ID: (Human) 51053