Rockland

Gli2 Polyclonal Antibody

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抗体检测数据 (3)

Gli2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Gli2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Gli2 Antibody (600-401-695) in IHC (P)

Rocklands Affinity Purified anti-mouse Gli-2 antibody was used at 10 µg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.

Gli2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Gli2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Gli2 Antibody in Western Blot (WB)

Product Image Gallery

Gli2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Rocklands Affinity Purified anti-mouse Gli-2 antibody was used at 10 µg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.

Gli2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Rocklands Affinity Purified anti-mouse Gli-2 antibody was used at 10 µg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.

Gli2 Antibody in Western Blot (WB) — Western blot using Rocklands Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 µg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (611-132-122) for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

产品信息

600-401-695

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

-

免疫组化（石蜡） (IHC (P))

2-20 µg/mL

-

酶联免疫吸附实验 (ELISA)

1:15,000-1:60,000

-

免疫沉淀 (IP)

1:500-1:2,000

-

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.02 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against mouse Gli-2 protein. A BLAST analysis was used to suggest cross-reactivity with Gli-2 from mouse and rat sources based on 100% sequence homology with the immunogen. Reactivity with Gli-2 from other sources is not known.

靶标信息

Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Tax helper protein; Zinc finger protein GLI2

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基因别名: AW546128; Gli2; Thp

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UniProt ID: (Mouse) Q0VGT2

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Entrez Gene ID: (Mouse) 14633

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding nucleic acid binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding DNA-binding transcription factor

negative regulation of transcription from RNA polymerase II promoter skeletal system development osteoblast differentiation in utero embryonic development kidney development morphogenesis of an epithelium chondrocyte differentiation osteoblast development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter smoothened signaling pathway pattern specification process axon guidance ventral midline development hindgut morphogenesis heart development cell proliferation positive regulation of cell proliferation regulation of smoothened signaling pathway epidermal cell differentiation dorsal/ventral pattern formation proximal/distal pattern formation floor plate formation spinal cord dorsal/ventral patterning ventral spinal cord development spinal cord motor neuron differentiation cerebellar cortex morphogenesis smoothened signaling pathway involved in ventral spinal cord interneuron specification smoothened signaling pathway involved in spinal cord motor neuron cell fate specification dorsal/ventral neural tube patterning neural tube development smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation spinal cord ventral commissure morphogenesis pituitary gland development cell differentiation lung development mammary gland development hindbrain development negative regulation of chondrocyte differentiation tube development odontogenesis of dentin-containing tooth embryonic digit morphogenesis positive regulation of neuron differentiation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract development developmental growth anatomical structure formation involved in morphogenesis neuron development branching morphogenesis of an epithelial tube anatomical structure development notochord regression head development prostatic bud formation mammary gland duct morphogenesis smoothened signaling pathway involved in dorsal/ventral neural tube patterning cellular response to organic cyclic compound cochlea morphogenesis cellular response to virus