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Invitrogen
Glucocorticoid receptor (NR3C1) Monoclonal Antibody (BuGR2), APC, eBioscience™
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Glucocorticoid receptor (NR3C1) Antibody (17-6189-82) in Flow
Mouse splenocytes were stained with CD3 Monoclonal Antibody, PE (Product # 12-0031-82), fixed and permeabilized with the Foxp3/Transcription factor staining buffer set (Product # 00-5523-00) and stained with 0.25 µg mouse IgG2a, kappa Isotype Control, APC (Product # 17-4724-81) (left) or BuGR2 Monoclonal Antibody, APC (right). Viable cells in the lymphocytes gate were identified with eBioscience™ Fixable Vi... View More
产品信息
17-6189-82
产品规格
种属反应
Mouse
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
BuGR2
抗原
Partially purified rat GR
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2811764
产品详细信息
Description: This BuGR2 monoclonal antibody reacts with mouse glucocorticoid receptor NR3CR1. This clone has been also reported to cross-react with human NR3CR1. This BuGR2 antibody is recommended to be used with the Foxp3/Transcription factor staining buffer set (Product # 00-5523-00).
Applications Reported: This BuGR2 antibody has been reported for use in flow cytometric analysis. This clone has been also reported to cross-react with Human, Non-human primate, Rabbit, Rat, Sheep, Xenopus, Yeast NR3CR1.
Applications Tested: This BuGR2 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
靶标信息
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Glucocorticoid receptor; glucocorticoid receptor 1; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; OTTHUMP00000222854; OTTHUMP00000222858
基因别名: GR; Grl; Grl-1; Grl1; Nr3c1
UniProt ID: (Mouse) P06537
Entrez Gene ID: (Mouse) 14815
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II regulatory region DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription coactivator activity
glucocorticoid receptor activity
steroid binding
protein binding
transcription factor binding
zinc ion binding
lipid binding
Hsp70 protein binding
receptor tyrosine kinase binding
heat shock protein binding
protein complex binding
glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity
hormone binding
protein homodimerization activity
sequence-specific DNA binding
metal ion binding
protein heterodimerization activity
protein dimerization activity
Hsp90 protein binding
steroid hormone binding
regulation of gluconeogenesis
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
glucocorticoid metabolic process
regulation of glucose metabolic process
positive regulation of glutamate secretion
chromatin modification
adrenal gland development
negative regulation of synaptic plasticity
regulation of glucocorticoid biosynthetic process
regulation of cell proliferation
maternal behavior
glucocorticoid receptor signaling pathway
negative regulation of vascular permeability
steroid hormone mediated signaling pathway
glucocorticoid mediated signaling pathway
positive regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
response to arsenic-containing substance
chromatin-mediated maintenance of transcription
mammary gland duct morphogenesis
positive regulation of dendritic spine development
positive regulation of cell growth involved in cardiac muscle cell development
cellular response to steroid hormone stimulus
cellular response to dexamethasone stimulus
negative regulation of glucocorticoid mediated signaling pathway
positive regulation of glucocorticoid receptor signaling pathway
