Invitrogen

Glypican 1 Polyclonal Antibody

Glypican 1 Antibody in Immunocytochemistry (ICC/IF)

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Glypican 1 Antibody (PA5-28055) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Glypican 1 was performed in MCF 7 cells fixed in ice cold MeOH for 5 min. Green: Glypican 1 Polyclonal Antibody (Product # PA5 28055) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Glypican 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Glypican 1 Antibody in Western Blot (WB)

Glypican 1 Antibody in Flow Cytometry (Flow)

Glypican 1 Antibody in Immunoprecipitation (IP)

Glypican 1 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-28055

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

流式细胞分析 (Flow)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1:1,000-1:10,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.71 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545531

产品详细信息

Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/mL Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/mL Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr).

Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。