Invitrogen

Glypican 1 Polyclonal Antibody

Glypican 1 Antibody in Flow Cytometry (Flow)

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Glypican 1 Antibody (PA5-47632) in Flow

Flow cytometric analysis of Glypican 1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-human Glypican 1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47632) at 10 µg/mL for 3 hours at room temperature. Cells were stained using a 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.

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产品信息

PA5-47632

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫细胞化学 (ICC/IF)

5-15 µg/mL

流式细胞分析 (Flow)

2.5 µg per million cells

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant human Glypican 1 Asp24-Ser530

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2608930

产品详细信息

In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse Glypican 1 is observed and less than 1% cross-reactivity with recombinant human (rh) Glypican 5 and rhGlypican 6 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。