Invitrogen

Glypican 1 Polyclonal Antibody

Glypican 1 Antibody in Western Blot (WB)

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Glypican 1 Antibody (PA5-88920) in WB

Western blot analysis of Glypican 1 in extracts of various cell lines. Samples were incubated with Glypican 1 Polyclonal antibody (Product # PA5-88920) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

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产品信息

PA5-88920

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 290-420 of human GPC1 (NP_0020722)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.4 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2805221

产品详细信息

Immunogen sequence: WRNLLDSMVL ITDKFWGTSG VESVIGSVHT WLAEAINALQ DNRDTLTAKV IQGCGNPKVN PQGPGPEEKR RRGKLAPRER PPSGTLEKLV SEAKAQLRDV QDFWISLPGT LCSEKMALST ASDDRCWNGM A; Positive Samples: NIH/3T3, Mouse brain, Rat brain; Cellular Location: Cell membrane, Endosome, Extracellular side, GPI-anchor, Lipid-anchor, extracellular space

靶标信息

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。