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Mouse IgM Secondary Antibody (31992) in Flow
Flow cytometry analysis of TRA-1-81 was performed on NCCIT cells. NCCIT cells were harvested, fixed and washed with PBS. Cells were incubated with 0.4 µg of TRA-1-60 monoclonal antibody (Product # MA1-024), followed by incubation with FITC-conjugated goat-anti-mouse IgM secondary antibody (Product # 31992), indicated by the shifted peak (blue line). To control for non-specific binding, cells were also incub... View More
产品信息
31992
应用
建议稀释比
免疫组化 (IHC)
1:50 - 1:200
免疫细胞化学 (ICC/IF)
1:50 - 1:200
流式细胞分析 (Flow)
1:50 - 1:200
免疫沉淀 (IP)
1:50 - 1:200
产品规格
种属反应
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Lyophilized
浓度
1.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_228328
靶标
IgM
抗体形式
Whole Antibody
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31992 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31992 reacts with the heavy chain of mouse IgM, but does not react against IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. The concentration of antibody and buffer salts will decrease to one-half of the original after addition of glycerol. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: ~ 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration). Product is stable for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
Country of Origin: USA
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
