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Invitrogen
F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC, eBioscience™
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Rabbit IgG (H+L) Secondary Antibody (11-4839-81) in Flow
Staining of mouse splenocytes with Anti-Mouse CD39 Serum followed by 1.0 µg of F (ab')2 Anti-Rabbit IgG FITC and subsequently with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD25 PE (Product # 12-0251-82). The histograms (right) demonstrates staining of CD39 on CD4+CD25. Cells (red histogram) and CD4+CD25+ cells (green histogram), as gated in the dot plot (left).
产品信息
11-4839-81
应用
建议稀释比
免疫组化 (IHC)
Assay-Dependent
免疫细胞化学 (ICC/IF)
1 µg/mL
流式细胞分析 (Flow)
1 µg/test
产品规格
种属反应
Rabbit
宿主/亚型
Goat
/ Ig
分类
Polyclonal
类型
Secondary Antibody
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1210845
靶标
IgG
抗体形式
F(ab')2 Fragment
产品详细信息
Description: The anti-rabbit IgG polyclonal antibody reacts with the heavy and light chains of rabbit IgG, with minimal cross reaction to human, mouse and rat serum proteins. Reactions to non-immunoglobulin rabbit serum proteins have not been detected.
Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis and immunocytochemistry to detect polyclonal rabbit antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For immunocytochemistry applications, this can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
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