Invitrogen

Granulins Polyclonal Antibody

Granulins Antibody in Immunocytochemistry (ICC/IF)

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Granulins Antibody (PA5-27275) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Granulins was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Granulins Polyclonal Antibody (Product # PA5-27275) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Granulins Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-27275

应用

建议稀释比

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免疫印迹 (WB)

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 278 and 585 of Human Granulins

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.43 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2544751

产品详细信息

Recommended positive controls: HepG2, mouse kidney, rat kidney.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

PCDGF is a glycoprotein and growth factor involved in the regulation of mitosis, early embryonic development, cell division, cell migration, cell survival, and hypothalamic differentiation. PCDGF is also known as progranulin because it is the precursor for smaller proteins known as epithelins or granulins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Progranulin sustains STAT3 hyper-activation and oncogenic function in colorectal cancer cells.

Molecular oncology

Laudisi F,Cherubini F,Di Grazia A,Dinallo V,Di Fusco D,Franzè E,Ortenzi A,Salvatori I,Scaricamazza S,Monteleone I,Sakamoto N,Monteleone G,Stolfi C

PA5-27275 was used in Immunocytochemistry-immunoflourescence to show that progranulin, a STAT3 cofactor, is upregulated in human colorectal cancer (CRC) as compared to nontumor tissue/cells and its expression correlates with STAT3 activation.

Tue Oct 01 00:00:00 UTC 2019

Progranulin sustains STAT3 hyper-activation and oncogenic function in colorectal cancer cells.

Molecular oncology

Laudisi F,Cherubini F,Di Grazia A,Dinallo V,Di Fusco D,Franzè E,Ortenzi A,Salvatori I,Scaricamazza S,Monteleone I,Sakamoto N,Monteleone G,Stolfi C

Tue Oct 01 00:00:00 UTC 2019

Progranulin sustains STAT3 hyper-activation and oncogenic function in colorectal cancer cells.

Molecular oncology

Laudisi F,Cherubini F,Di Grazia A,Dinallo V,Di Fusco D,Franzè E,Ortenzi A,Salvatori I,Scaricamazza S,Monteleone I,Sakamoto N,Monteleone G,Stolfi C

Tue Oct 01 00:00:00 UTC 2019

Progranulin sustains STAT3 hyper-activation and oncogenic function in colorectal cancer cells.

Molecular oncology

Laudisi F,Cherubini F,Di Grazia A,Dinallo V,Di Fusco D,Franzè E,Ortenzi A,Salvatori I,Scaricamazza S,Monteleone I,Sakamoto N,Monteleone G,Stolfi C

Tue Oct 01 00:00:00 UTC 2019

Granulin, a novel STAT3-interacting protein, enhances STAT3 transcriptional function and correlates with poorer prognosis in breast cancer.

Genes & cancer

Yeh JE,Kreimer S,Walker SR,Emori MM,Krystal H,Richardson A,Ivanov AR,Frank DA

PA5-27275 was used in western blot to identify novel STAT3-interacting proteins in breast cancer cells

Sun Mar 01 00:00:00 UTC 2015

生物信息学

蛋白别名: Acrogranin; Epithelin precursor; Glycoprotein of 88 Kda; GP88; Granulin precursor; granulin-epithelin; PC cell-derived growth factor; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

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基因别名: CLN11; GEP; GP88; GRN; PCDGF; PEPI; PGRN

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UniProt ID: (Human) P28799

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Entrez Gene ID: (Human) 2896

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功能

cytokine activity protein binding growth factor activity poly(A) RNA binding

参与通路

signal transduction response to estradiol chondrocyte proliferation synaptic vesicle endocytosis male mating behavior positive regulation of dendritic spine development neural precursor cell proliferation