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Granzyme B Antibody (13-8822-82) in WB
BALB/c splenocytes were stimulated with Mouse IL-2 Recombinant protein (100 ng/mL) (Product # 14-8021-64) for 3 days. Subsequently, splenocyte lysates were loaded at 1x10e6 cells/lane, probed with 1 µg/mL Anti-Mouse Granzyme B Purified and revealed with HRP anti-rat IgG.
产品信息
13-8822-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
16G6
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1603282
产品详细信息
Description: The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis.
For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (Product # 00-5523). Other buffers may yield varying results.
Applications Reported: This 16G6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: The biotinylated 16G6 antibody has been tested by immunoblotting (WB); a recommended starting concentration is 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CCP1; CTLA-1; Cytotoxic cell protease 1; Cytotoxic T lymphocyte associated serine esterase 1; Fragmentin-2; Granzyme B(G,H); GranzymeB; Human lymphocyte protein (Hlp); OTTHUMP00000028189
基因别名: AI553453; CCP-1/C11; CCP1; Ctla-1; Ctla1; GZB; Gzmb
UniProt ID: (Mouse) P04187
Entrez Gene ID: (Mouse) 14939
