Invitrogen

Grim-19 Monoclonal Antibody (1A8), eBioscience™

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Grim-19 Antibody (14-9937-82) in WB

Immunoblotting of reduced HeLa cell lysate with 5 µg/mL of Anti-Grim-19 Purified antibody. Bands were visualized using Anti-Mouse IgG HRP.

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产品信息

14-9937-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

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免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

Assay-Dependent

流式细胞分析 (Flow)

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体外检测 (IV)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

1A8

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10597591

产品详细信息

Description: This 1A8 monoclonal antibody reacts with human, mouse, and rat Grim-19. This 16 kDa protein is expressed in many human tissues, especially in the heart and skeletal muscle. Lower expression levels of Grim-19 can be observed in the liver, kidney, and brain. Originally described as a nuclear protein, reports indicate that Grim-19 is primarily localized to mitochondria as an essential component of complex I of the electron transport chain. Grim-19 expression can be induced by interferon-beta and all-trans-retinoic acid in tumor cell lines, leading to cell death. Finally, Grim-19 has been shown to bind the transactivation domain of STAT3 and inhibit its transcriptional activity. The molecular mechanisms of how Grim-19 regulates STAT3 within the cell remains under active investigation.

Applications Reported: This 1A8 antibody has been reported for use in immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This 1A8 antibody has been tested by immunoblotting of HeLa cell lysate. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

A novel gene associated with Retinoid-IFN-induced Mortality (GRIM) GRIM-19 gene was identified. Antisense expression of GRIM-19 confers a strong resistance against IFN/RA-induced death by reducing the intracellular levels of GRIM-19 protein. Overexpression of GRIM-19 enhances cell death in response to IFN/RA. GRIM-19 is primarily a nuclear protein whose expression is induced by the IFN/RA combination. These data indicate that GRIM-19 is a novel cell death-regulatory molecule.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Discrete viral E2 lysine residues and scavenger receptor MARCO are required for clearance of circulating alphaviruses.

eLife

Carpentier KS,Davenport BJ,Haist KC,McCarthy MK,May NA,Robison A,Ruckert C,Ebel GD,Morrison TE

14-9937-82 was used in Flow Cytometry to reveal a previously unrecognized innate immune pathway that controls alphavirus viremia and dissemination in vertebrate hosts, ultimately influencing disease severity and likely transmission efficiency.

Wed Oct 09 00:00:00 UTC 2019

Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.

PLoS pathogens

Pyle CJ,Uwadiae FI,Swieboda DP,Harker JA

14-9937-82 was used in Flow Cytometry to show that during respiratory virus infection the prototypic inflammatory cytokine IL-6 is a critical anti-inflammatory regulator of viral induced immunopathology in the respiratory tract through its induction of IL-27.

Fri Sep 01 00:00:00 UTC 2017

Disulfide HMGB1 derived from platelets coordinates venous thrombosis in mice.

Blood

Stark K,Philippi V,Stockhausen S,Busse J,Antonelli A,Miller M,Schubert I,Hoseinpour P,Chandraratne S,von Brühl ML,Gaertner F,Lorenz M,Agresti A,Coletti R,Antoine DJ,Heermann R,Jung K,Reese S,Laitinen I,Schwaiger M,Walch A,Sperandio M,Nawroth PP,Reinhardt C,Jäckel S,Bianchi ME,Massberg S

14-9937 was used in Immunofluorescence in immunohistochemistry on frozen tissues to identify blood-derived high-mobility group box 1 protein to be a master regulator of the prothrombotic cascade involving platelets and myeloid leukocytes fostering occlusive deep venous thrombosis formation.

Thu Nov 17 00:00:00 UTC 2016

Immune signatures of protective spleen memory CD8 T cells.

Scientific reports

Brinza L,Djebali S,Tomkowiak M,Mafille J,Loiseau C,Jouve PE,de Bernard S,Buffat L,Lina B,Ottmann M,Rosa-Calatrava M,Schicklin S,Bonnefoy N,Lauvau G,Grau M,Wencker M,Arpin C,Walzer T,Leverrier Y,Marvel J

14-9937 was used in Flow cytometry/Cell sorting to identify a range of qualities uniquely associated with protective memory cells.

Thu Nov 24 00:00:00 UTC 2016

Pneumolysin expression by streptococcus pneumoniae protects colonized mice from influenza virus-induced disease.

Virology

Wolf AI,Strauman MC,Mozdzanowska K,Williams KL,Osborne LC,Shen H,Liu Q,Garlick D,Artis D,Hensley SE,Caton AJ,Weiser JN,Erikson J

14-9937-82 was used in Flow Cytometry to investigate severity of influenza virus response with respiratory microbe presence.

Fri Aug 01 00:00:00 UTC 2014

Anatomically restricted synergistic antiviral activities of innate and adaptive immune cells in the skin.

Cell host & microbe

Hickman HD,Reynoso GV,Ngudiankama BF,Rubin EJ,Magadán JG,Cush SS,Gibbs J,Molon B,Bronte V,Bennink JR,Yewdell JW

14-9937 was used in Flow cytometry/Cell sorting to explore the spatiotemporal organization between antiviral immune cells and vaccinia virus-infected murine cells.

Wed Feb 13 00:00:00 UTC 2013

Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo.

The Journal of experimental medicine

von Brühl ML,Stark K,Steinhart A,Chandraratne S,Konrad I,Lorenz M,Khandoga A,Tirniceriu A,Coletti R,Köllnberger M,Byrne RA,Laitinen I,Walch A,Brill A,Pfeiler S,Manukyan D,Braun S,Lange P,Riegger J,Ware J,Eckart A,Haidari S,Rudelius M,Schulz C,Echtler K,Brinkmann V,Schwaiger M,Preissner KT,Wagner DD,Mackman N,Engelmann B,Massberg S

14-9937 was used in in vivo experiments to investigate a novel mouse model of deep vein thrombosis as a useful tool to study the sequence of disease events.

Mon Apr 09 00:00:00 UTC 2012

The cell death regulator GRIM-19 is an inhibitor of signal transducer and activator of transcription 3.

Proceedings of the National Academy of Sciences of the United States of America

Zhang J,Yang J,Roy SK,Tininini S,Hu J,Bromberg JF,Poli V,Stark GR,Kalvakolanu DV

Published figure using Grim-19 monoclonal antibody (Product # 14-9937-82) in Western Blot

Tue Aug 05 00:00:00 UTC 2003

生物信息学

蛋白别名: Cell d; Cell death regulatory protein GRIM-19; cell death-regulatory protein GRIM19; CI-B16.6; complex I B16.6 subunit; Complex I-B16.6; FLJ58045; FLJ59191; gene associated with retinoic and IFN-induced mortality 19 protein; Gene associated with retinoic and interferon-induced mortality 19 protein; gene associated with retinoic-interferon-induced mortality 19 protein; genes associated with retinoid-IFN-induced mortality 19; GRIM-19; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH-ubiquinone oxidoreductase B16.6 subunit

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基因别名: 2700054G14Rik; AU022060; B16.6; CDA016; CGI-39; GRIM-19; GRIM19; NDUFA13

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UniProt ID: (Human) Q9P0J0, (Mouse) Q9ERS2

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Entrez Gene ID: (Rat) 100911483, (Human) 51079, (Mouse) 67184

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功能

NADH dehydrogenase activity protein binding ATP binding NADH dehydrogenase (ubiquinone) activity dehydrogenase oxidoreductase metabolite interconversion enzyme

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