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H3.3K79me2 Antibody (PA5-112440) in WB
Western Blot analysis of H3.3K79me2 in multiple sample types using a H3.3K79me2 polyclonal antibody (Product # PA5-112440) at a dilution of 1:1000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:40,000. Sample types that were tested include: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate .
产品信息
PA5-112440
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide sequence around site of Di-methyl-Lys (79) derived from Human Histone H3.3
偶联物
形式
Liquid
浓度
0.15 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2867175
靶标信息
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Histone H3.3
基因别名: H3-3A; H3-3B; H3.3A; H3.3B; H3F3; H3F3A; H3F3B; PP781
UniProt ID: (Human) P84243
Entrez Gene ID: (Human) 3020
chromatin silencing at rDNA
nucleosome assembly
DNA replication-independent nucleosome assembly
brain development
blood coagulation
response to hormone
positive regulation of cell growth
gene silencing by RNA
telomere organization
cellular protein metabolic process
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
