Invitrogen

H4K20me3 Polyclonal Antibody

H4K20me3 Antibody in Immunocytochemistry (ICC/IF)

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H4K20me3 Antibody (PA5-85527) in ICC/IF

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (Product # PA5-85527) diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by (Product # MA5-31466) diluted at 1:500. Blue: Hoechst 33342 staining.

H4K20me3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-85527

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

点杂交 (DB)

1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me3 (trimethyl Lys20).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2792667

产品详细信息

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Xenopus laevis (100%).

Positive Control: HeLa, HeLa nuclear.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K20; H4K20; H4Lys20me3; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4A; Histone H4K20me3; histone IV, family 2; methyl Histone 3; methyl Histone H3; Tri-methyl H4; Tri-methyl Histone H4; Tri-Methyl-H4 K20; Tri-Methyl-H4 Lys20; Tri-Methyl-Histone H4 K20; Tri-Methyl-Histone H4 Lys20; Trimethyl H4; Trimethyl Histone H4

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基因别名: dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652

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UniProt ID: (Human) P62805, (Mouse) Q811M0, (Mouse) P62806