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HA Epitope Tag Antibody (600-441-384) in WB
Western Blot of Rabbit anti-HA DyLight™ 488 Conjugated Antibody. Lane 1: 12 Epitope Tag Protein Marker Lysate. Lane 2: None. Load: 10 µL. Primary antibody: None. Secondary antibody: DyLight™ 488 rabbit secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 69 kDa, 69 kDa for HA. Other band(s): None.
产品信息
600-441-384
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 114-122 (Y-P-Y-D-V-P-D-Y-A-G) of hemagglutinin influenza conjugated to KLH using maleimide.
偶联物
激发/发射光谱
492/519 nm
查看光谱
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
内含物
0.01% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the HA epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the HA epitope tag (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-Gly) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
靶标信息
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
