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HA Tag Antibody (14-6756-81) in ICC/IF
Immunofluorescent analysis of HA Tag was performed using HA-H3 construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Polyclonal Antibody, eBioscience™ (Product # 14-67-5663) at 1 µg/mL and Histone H3 Monoclonal Antibody (865R2) Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Ra... View More
产品信息
14-6756-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with BSA, 0.02% gelatin
内含物
0.1% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_468301
产品详细信息
Description: The polyclonal antibody reacts with HA tag. One of the more established epitope tags is the HA tag, which is a nine amino acid peptide sequence (YPYDVPDYA) present in the human influenza virus hemagglutinin protein. The HA tag has been successfully used in a variety of applications, including Western blotting, immunoprecipitation, and immunfluorescence studies.
Applications Reported: Purified Anti-HA tag poly has been reported for use in immunoprecipitation, and immunoblotting (WB).
Applications Tested: Purified Anti-HA tag poly has been tested by immunoblotting (WB). (1:200 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin
