Invitrogen

HADH Polyclonal Antibody

HADH Antibody in Immunocytochemistry (ICC/IF)

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HADH Antibody (PA5-28203) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of HADH was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HADH Polyclonal Antibody (Product # PA5-28203) diluted at 1:500. Blue: Hoechst 33342 staining.

HADH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HADH Antibody in Immunoprecipitation (IP)

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产品信息

PA5-28203

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

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免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein fragment corresponding to a region within amino acids 1 and 263 of Human HADH

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545679

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, GL261, C8D30, PC-12.

Predicted reactivity: Mouse (92%), Rat (92%), Xenopus laevis (82%), Pig (95%), Chicken (80%), Rhesus Monkey (98%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Short-Term High-Fat Feeding Does Not Alter Mitochondrial Lipid Respiratory Capacity but Triggers Mitophagy Response in Skeletal Muscle of Mice.

Frontiers in endocrinology

Ehrlicher SE,Stierwalt HD,Newsom SA,Robinson MM

PA5-28203 was used in Western Blotting to investigate changes in mitophagy and respiratory chains in response to high fat diet in mice.

Fri Jan 07 00:00:00 UTC 2022

Short-Term High-Fat Feeding Does Not Alter Mitochondrial Lipid Respiratory Capacity but Triggers Mitophagy Response in Skeletal Muscle of Mice.

Frontiers in endocrinology

Ehrlicher SE,Stierwalt HD,Newsom SA,Robinson MM

PA5-28203 was used in Western Blotting to investigate changes in mitophagy and respiratory chains in response to high fat diet in mice.

Fri Jan 07 00:00:00 UTC 2022

Robust intrinsic differences in mitochondrial respiration and H₂O₂ emission between L6 and C2C12 cells.

American journal of physiology. Cell physiology

Robinson MM,Sather BK,Burney ER,Ehrlicher SE,Stierwalt HD,Franco MC,Newsom SA

PA5-28203 was used in Western Blotting to characterize and compare intrinsic mitochondrial metabolism between L6 and C2C12 cells.

Thu Aug 01 00:00:00 UTC 2019

Long-term rates of mitochondrial protein synthesis are increased in mouse skeletal muscle with high-fat feeding regardless of insulin-sensitizing treatment.

American journal of physiology. Endocrinology and metabolism

Newsom SA,Miller BF,Hamilton KL,Ehrlicher SE,Stierwalt HD,Robinson MM

PA5-28203 was used in Western Blotting to investigate the impact of changes in insulin sensitivity on long-term rates of mitochondrial protein synthesis as a mechanism for changes to mitochondrial respiration in skeletal muscle in mice.

Wed Nov 01 00:00:00 UTC 2017

生物信息学

蛋白别名: HCDH; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; hydroxylacyl-Coenzyme A dehydrogenase short chain; hydroxylacyl-Coenzyme A dehydrogenase, short chain; hydroxylacyl-Coenzyme A dehydrogenase-dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; M/SCHAD; medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; MGC8392; short chain 3-hydroxyacyl-CoA dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; testis secretory sperm-binding protein Li 203a

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基因别名: AA409008; AU019341; AW742602; HAD; HAD1; HADH; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD

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UniProt ID: (Human) Q16836, (Mouse) Q61425, (Rat) Q9WVK7

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Entrez Gene ID: (Human) 3033, (Mouse) 15107, (Rat) 113965

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功能

3-hydroxyacyl-CoA dehydrogenase activity NAD+ binding oxidoreductase activity

参与通路

fatty acid beta-oxidation response to activity response to insulin response to drug negative regulation of insulin secretion lipid metabolic process fatty acid metabolic process oxidation-reduction process response to hormone