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Invitrogen
HADH Polyclonal Antibody
FIGURE: 1 / 11
HADH Antibody (PA5-31157) in ICC/IF
Immunofluorescence analysis of HADH was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HADH Polyclonal Antibody (Product # PA5-31157) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More
Product Details
PA5-31157
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 92 and 314 of Human HADH
Conjugate
Form
Liquid
Concentration
0.49 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548631
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, rat heart, rat liver.
Predicted reactivity: Mouse (90%), Rat (91%), Xenopus laevis (85%), Pig (92%), Chicken (81%), Rhesus Monkey (96%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HCDH; Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; hydroxylacyl-Coenzyme A dehydrogenase short chain; hydroxylacyl-Coenzyme A dehydrogenase, short chain; hydroxylacyl-Coenzyme A dehydrogenase-dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; M/SCHAD; medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; MGC8392; short chain 3-hydroxyacyl-CoA dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; testis secretory sperm-binding protein Li 203a
Gene Aliases: AA409008; AU019341; AW742602; HAD; HAD1; HADH; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD
UniProt ID: (Human) Q16836, (Mouse) Q61425, (Rat) Q9WVK7
Entrez Gene ID: (Human) 3033, (Mouse) 15107, (Rat) 113965
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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