Invitrogen

HAT1 Polyclonal Antibody

HAT1 Antibody in Immunocytochemistry (ICC/IF)

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HAT1 Antibody (PA5-29174) in ICC/IF

HAT1 Polyclonal Antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAT1 protein stained by HAT1 Polyclonal Antibody [N1C3-2] (Product # PA5-29174) diluted at 1:500. Blue: Hoechst 33342 staining.

HAT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HAT1 Antibody in Immunoprecipitation (IP)

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产品信息

PA5-29174

应用

建议稀释比

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免疫印迹 (WB)

1:5,000-1:20,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human HAT1 Recombinant protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.98 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2546650

产品详细信息

Recommended positive controls: U87-MG.

Predicted reactivity: Mouse (92%), Rat (95%), Chicken (83%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。