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HBEGF Antibody (500-P329BT-25UG) in ELISA
Sandwich ELISA: To detect Human HB-EGF by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of HBEGF Polyclonal Antibody, Biotin (Product # 500-P329BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech HBEGF Polyclonal Antibody (Product # 500-P329-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human HB-EGF.
产品信息
500-P329BT-25UG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived, 9.7 kDa Recombinant Human HB-EGF
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930444
产品详细信息
AA Sequence of recombinant protein: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HB-EGF. Anti-Human HB-EGF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human HB-EGF by sandwich ELISA (using 100 µL/well), a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human HB-EGF (500-P329) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human HB-EGF.
Western Blot: To detect Human HB-EGF by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human HB-EGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P329BT-1MG will be provided as 2 x 500 µg
靶标信息
HBEGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function. HBEGF promotes smooth muscle cell proliferation. It may be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. It also acts as a diphtheria toxin receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); hb egf; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; Proheparin-binding EGF-like growth factor
基因别名: DTR; DTS; DTSF; HBEGF; HEGFL
UniProt ID: (Human) Q99075
Entrez Gene ID: (Human) 1839
MAPK cascade
signal transduction
epidermal growth factor receptor signaling pathway
muscle organ development
regulation of heart contraction
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
positive regulation of cell growth
positive regulation of cell migration
wound healing, spreading of epidermal cells
ERBB2 signaling pathway
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
positive regulation of smooth muscle cell proliferation
negative regulation of elastin biosynthetic process
positive regulation of keratinocyte migration
positive regulation of protein kinase B signaling
cell chemotaxis
positive regulation of wound healing
regulation of cell motility
