Invitrogen

HCK Polyclonal Antibody

HCK Antibody in Immunocytochemistry (ICC/IF)

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HCK Antibody (PA5-105729) in ICC/IF

Immunocytochemistry analysis of HCK in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HCK polyclonal antibody (Product # PA5-105729) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

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产品信息

PA5-105729

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human HCK(Accession P08631), corresponding to amino acid residues N496-P526.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2853912

产品详细信息

Antibody detects endogenous levels of total Hck.

靶标信息

Hematopoietic cell kinase (HCK) is a SRC-related kinase expressed in lymphoid and hematopoietic cells and may play a role in some forms of leukemia. (HCK Alias: DKFZp686D0638). HCK is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: B cell/myeloid kinase; B-cell/myeloid kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK; p59-HCK/p60-HCK; p59Hck; p61Hck; Tyrosine-protein kinase HCK

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基因别名: AI849071; Bmk; HCK; JTK9; p59Hck; p61Hck

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UniProt ID: (Human) P08631, (Mouse) P08103

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Entrez Gene ID: (Human) 3055, (Mouse) 15162

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功能

protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding protein binding ATP binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

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