Invitrogen

HCLS1 Polyclonal Antibody

HCLS1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

HCLS1 Antibody (PA5-30057) in ICC/IF

Immunofluorescence analysis of HCLS1 was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HCLS1 Polyclonal Antibody (Product # PA5-30057, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000), f... View More

HCLS1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

HCLS1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of HCLS1 was observed in NK-92, Jurkat, THP-1, Raji in comparison with low expressing cell lines such as HeLa, SK-O-V3 and Hep G2 using HCLS1 Polyclonal Antibody (Product # PA5-30057) in Western Blot. {RE}

产品信息

PA5-30057

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

1:200

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 221 of Human HCLS1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.95 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547531

产品详细信息

Recommended positive controls: 293T, A431, Jurkat, Raji.

Predicted reactivity: Mouse (95%), Rat (95%), Chicken (82%), Rhesus Monkey (98%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProt]

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。