Invitrogen

HCLS1 Recombinant Rabbit Monoclonal Antibody (PSH01-12)

HCLS1 Antibody in Immunocytochemistry (ICC/IF)

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HCLS1 Antibody (MA5-49608) in ICC/IF

Immunofluorescence analysis of HCLS1 was performed using 70% confluent log phase Raji and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HCLS1 Rabbit Recombinant Monoclonal Antibody (PSH01-12) (Product # MA5-49608 ) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, ... View More

HCLS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HCLS1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of HCLS1 was observed in NK-92, THP-1, Daudi, Raji in comparison with low expressing cell lines such as HeLa, SK-O-V3 and Hep G2 using HCLS1 Rabbit Recombinant Monoclonal Antibody (PSH01-12) (Product # MA5-49608) in Western Blot. {RE}

产品信息

MA5-49608

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:1,000-1:5,000

免疫细胞化学 (ICC/IF)

1:100-1:250

流式细胞分析 (Flow)

1:500-1:1,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH01-12

抗原

Recombinant protein within human hcls1 aa 201-450/486

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3092879

产品详细信息

Sequence Similarities: 70% Mouse.

Tissue Specificity: Expressed only in tissues and cells of hematopoietic origin. Expressed in early stage of myeloid and erythroid differentiation.

Positive Control: Ramos cell lysate, Daudi cell lysate, Raji cell lysate, Jurkat cell lysate, HL-60 cell lysate, human lung carcinoma tissue, human liver tissue, HL-60, Jurkat.

Subcellular Location: Membrane, Cytoplasm, Mitochondrion.

Predicted band size: 54 kDa.

靶标信息

Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。