Invitrogen

HCN1 Monoclonal Antibody (RTQ 7C3), eBioscience™

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HCN1 Antibody (14-5691-82) in WB

Western Blot analysis was performed on whole cell extracts of Rat Brain (Lane 1), Mouse Brain (Lane 2) and HEK-293T(Lane 3) cells. The proteins were separated on a 4-12% Nupage Bis-Tris Gel (Product # NP0336BOX) using MOPS SDS Running Buffer (Product # NP0001-02). Protein transfer to a PVDF membrane (Product # IB24002) was achieved using the iBlot 2 system. Subsequently, the membrane was blocked with 5% milk and probed overnight at 4 C with 1.0 µg/mL Anti-Mouse/Rat HCN1 Monoclonal Antibody (clone RTQ-7C3), followed by Goat anti-Rat IgG Sec... View More

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HCN1 Antibody — Western blot analysis of cellular lysates of rat brain, mouse brain, mouse heart and mouse kidney. As expected based on known RNA expression pattern clone RTQ-7C3 detects a band of ~110 kDa corresponding to HCN1 in rat brain and mouse brain, but not in other lysates. {RE}

产品信息

14-5691-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1.0 µg/mL

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

RTQ 7C3

抗原

aa 650 - 685 of rat HCN1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2802194

产品详细信息

Description: This RTQ 7C3 monoclonal antibody recognizes mouse and rat HCN1. Clone RTQ 7C3 detects a ~110 kDa band corresponding to HCN1 by Western Blot.

Applications Reported: This RTQ 7C3 antibody has been reported for use in western blot.

Applications Tested: This RTQ 7C3 antibody has been tested by western blot of mouse and rat tissue lysates. This may be used at less than or equal to 1 µg/mL.

靶标信息

HCN1 includes Hyperpolarization-activated cation channels of the HCN gene family, which contribute to spontaneous rhythmic activity in both heart and brain. HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BCNG-1; Brain cyclic nucleotide-gated channel 1; HAC-2; Hyperpolarization-activated cation channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

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基因别名: Bcng1; C630013B14Rik; HAC2; Hcn1

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UniProt ID: (Mouse) O88704, (Rat) Q9JKB0

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Entrez Gene ID: (Mouse) 15165, (Rat) 84390

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功能