Invitrogen

HCN2 Monoclonal Antibody (QQA 4A6), eBioscience™

HCN2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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HCN2 Antibody (14-9692-82) in IHC (F)

Immunohistochemical analysis of HCN2 in a mouse frozen coronal brain section. Sample was incubated with HCN2 monoclonal antibody QQA 4A6 (40 ug/mL) for 1 hour at RT and followed by Goat Anti-Rat HRP and visualized with DAB Chromogen. Nuclei were counterstained with Mayer’s Hematoxylin.

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HCN2 Antibody — Western blot analysis of the indicated organ lysates. As expected based on known RNA expression patterns clone QQA 4A6 detects a primary band of ~95 kDa corresponding to HCN2 in mouse and rat brain or spinal cord lysates, but not in other tissues. Residual heavy and light chains of antibody (50 and 25 kDa, respectively) can be seen in the lanes corresponding to the rat tissues. Anti-GAPDH HRP was used as a gel loading control. {RE}

产品信息

14-9692-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

免疫组化（冰冻） (IHC (F))

40 µg/mL

产品规格

种属反应

Mouse, Rat

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

QQA 4A6

抗原

Peptide corresponding to aa676-710

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_2811746

产品详细信息

Description: This QQA 4A6 monoclonal antibody recognizes mouse and rat HCN2. QQA 4A6 detects a ~95 kDa band corresponding to HCN2 by Western Blot. An off target band of ~80 kDa is detected in cell line models.

Applications Reported: This QQA 4A6 antibody has been reported for use in western blot and immunohistochemical staining.

Applications Tested: This QQA 4A6 antibody has been tested by western blot and may be used at less than or equal to 1.0 µg/mL The QQA 4A6 antibody has been tested by immunohistochemistry of frozen mouse tissue sections and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Hyperpolarization-activated cation channels of the HCN gene family such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. HCN2 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 play an important role for motor learning and neuronal integration by cerebellar Purkinje cells; as well as, shaping autonomous activity of single neurons and the periodicity of network oscillations. The HCN1 expression is highly enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. HCN2 is highly abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. These variations in regional specificity of HCN channels could generate important differences in neuronal pacemaker activity across brain systems.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BCNG-2; Brain cyclic nucleotide-gated channel 2; HAC-1; Hyperpolarization-activated cation channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

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基因别名: BCNG2; HAC1; Hcn2; trls

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UniProt ID: (Mouse) O88703, (Rat) Q9JKA9

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Entrez Gene ID: (Mouse) 15166, (Rat) 114244

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功能