Invitrogen

HCN4 Monoclonal Antibody (N114/10), APC

HCN4 Antibody in Immunocytochemistry (ICC/IF)

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HCN4 Antibody (MA5-45431) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with HCN4 monoclonal antibody (Product # MA5-45431) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 antibody (D) Composite.

HCN4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-45431

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:100

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

Assay-dependent

Antibody Microarray (AM)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rabbit, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N114/10

抗原

Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4

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偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2931885

产品详细信息

1 µg/mL of MA5-45431 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 130kDa. No cross-reactivity against other HCNs.

靶标信息

HCN4 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BCNG-3; Brain cyclic nucleotide-gated channel 3; HAC-4; hyperpolarization activated cation channel; hyperpolarization activated cyclic nucleotide-gated cation channel 4; hyperpolarization activated cyclic nucleotide-gated potassium channel 4; Hyperpolarization-activated cation channel 4; hyperpolarization-activated, cyclic nucleotide-gated K+ 3; hyperpolarization-activated, cyclic nucleotide-gated K+ 4; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

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基因别名: Bcng3; HAC4; Hcn3; HCN4; SSS2