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HDAC3 Antibody (PA1-862) in ICC/IF
Immunofluorescent analysis of HDAC3 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC3 polyclonal antibody (Product # PA1-862) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
产品信息
PA1-862
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Hamster,
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2118393
产品详细信息
PA1-862 detects histone deacetylase 3 (HDAC3) from canine, hamster, human and rat tissues and cells.
PA1-862 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~48 kDa protein representing HDAC3 from HeLa cell lysate.
The PA1-862 immunogen is a synthetic peptide corresponding to residues C D(415) G D H D N D K E S D V E I(428) of human HDAC3. This immunizing peptide (Cat. # PEP-071) is available for use in neutralization and control experiments.
靶标信息
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HD3; Histone deacetylase 3; histone deacetylase 3-like protein; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45
基因别名: HD3; HDAC3; RPD3; RPD3-2
UniProt ID: (Human) O15379, (Rat) Q6P6W3
Entrez Gene ID: (Dog) 478040, (Human) 8841, (Rat) 84578, (Chinese hamster) 100752639
chromatin binding
transcription corepressor activity
histone deacetylase activity
protein binding
transcription factor binding
enzyme binding
cyclin binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
histone deacetylase binding
NF-kappaB binding
DNA binding
chromatin DNA binding
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
GTPase binding
NAD-dependent histone deacetylase activity (H3-K18 specific)
negative regulation of transcription from RNA polymerase II promoter
positive regulation of protein phosphorylation
transcription, DNA-templated
protein deacetylation
regulation of mitotic cell cycle
negative regulation of myotube differentiation
histone deacetylation
regulation of protein stability
response to nutrient levels
positive regulation of TOR signaling
negative regulation of interleukin-1 production
negative regulation of tumor necrosis factor production
circadian regulation of gene expression
regulation of multicellular organism growth
response to drug
positive regulation of transcription factor import into nucleus
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of neuron apoptotic process
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JNK cascade
negative regulation of protein export from nucleus
spindle assembly
histone H3-K9 modification
histone H3 deacetylation
histone H4 deacetylation
cellular response to mechanical stimulus
cellular response to parathyroid hormone stimulus
cellular response to fluid shear stress
response to dexamethasone
positive regulation of type B pancreatic cell apoptotic process
negative regulation of cardiac muscle cell differentiation
circadian rhythm
chromatin modification
negative regulation of apoptotic process
cellular lipid metabolic process
