Invitrogen

HDAC3 Recombinant Rabbit Monoclonal Antibody (8H2L16)

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HDAC3 Antibody (702440) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), C2C12 (Lane 2), COS-7 (Lane 3), Hep G2 (Lane 4), A-431 (Lane 5), MCF-7 (Lane 6) and tissue extracts of Mouse Brain (Lane 7) and Rat Brain (Lane 8). The blots were probed with Anti-HDAC3 Recombinant Rabbit Monoclonal Antibody (Product # 702440, 1 µg/mL). A 49 kDa band corresponding to HDAC3 was observed across the cell lines and tissues tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondar... View More

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HDAC3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with HDAC3 siRNA and decrease in signal intensity was observed in Western blot application (Fig a) using Anti-HDAC3 Recombinant Rabbit Monoclonal Antibody (Product # 702440). Densitometric analysis of this Western blot is shown in histogram (Fig b). {KD}

产品信息

702440

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

产品规格

种属反应

Cynomolgus monkey, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8H2L16

抗原

Protein corresponding to human HDAC3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2689475

产品详细信息

This antibody is predicted to react with Pig, Rat, Rabbit

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC3 belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: HD3; HDAC3 splicing HDAC3alpha; HDAC3 splicing HDAC3beta; HDAC3 splicing HDAC3delta; HDAC3 splicing HDAC3gamma; Histone deacetylase 3; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45

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基因别名: AW537363; HD3; HDAC3; RPD3; RPD3-2

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UniProt ID: (Human) O15379, (Mouse) O88895, (Rat) Q6P6W3