Invitrogen

HDAC9 Polyclonal Antibody

HDAC9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HDAC9 Antibody (PA5-90264) in IHC (P)

Immunohistochemistry analysis of HDAC9 in paraffin-embedded mouse intestine. Samples were incubated with HDAC9 Polyclonal antibody (Product # PA5-90264) using a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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产品信息

PA5-90264

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC9 (NP_4780561)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2805993

产品详细信息

Positive Samples: HeLa, K562; Cellular Location: Nucleus

Immunogen sequence: MHSMISSVDV KSEVPVGLEP ISPLDLRTDL RMMMPVVDPV VREKQLQQEL LLIQQQQQIQ KQLLIAEFQK QHENLTRQHQ AQLQEHIKEL LAIKQQQELL

靶标信息

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta and pancreas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: DKFZp779K1053; HD7; HD7b; HD9; hdac-9; histone deacetylase 4/5-related protein; Histone deacetylase 7B; Histone deacetylase 9; Histone deacetylase-related protein; MEF-2 interacting transcription repressor (MITR) protein; MEF2-interacting transcription repressor MITR

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基因别名: AV022454; D030072B18Rik; HD7; HD7B; HD9; HDAC; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; KIAA0744; MITR; mKIAA0744; RGD1310748; RGD1563092

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UniProt ID: (Human) Q9UKV0, (Mouse) Q99N13

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Entrez Gene ID: (Human) 9734, (Rat) 687001, (Mouse) 79221

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功能