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HEC1 Antibody (PA5-102765) in ICC/IF
Immunocytochemistry analysis of HEC1 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with HEC1 polyclonal antibody (Product # PA5-102765) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-102765
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NDC80(Accession O14777), corresponding to amino acid residues Y611-E642.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2852156
产品详细信息
Antibody detects endogenous levels of total NDC80.
靶标信息
Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HEC-1; HEC2; HEC4; Highly expressed in cancer protein; highly expressed in cancer, rich in leucine heptad repeats; HsHec1; kinetochore associated 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; NDC80 homolog, kinetochore complex component; NDC80 kinetochore complex component homolog; Retinoblastoma-associated protein HEC
基因别名: 2610020P18Rik; AV139730; HEC; HEC1; HsHec1; hsNDC80; KNTC2; NDC80; TID3
UniProt ID: (Human) O14777, (Mouse) Q9D0F1
Entrez Gene ID: (Human) 10403, (Rat) 301701, (Mouse) 67052
mitotic sister chromatid segregation
establishment of mitotic spindle orientation
mitotic spindle organization
chromosome segregation
sister chromatid cohesion
mitotic nuclear division
attachment of spindle microtubules to kinetochore
cell division
attachment of mitotic spindle microtubules to kinetochore
cell cycle
