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HEY1 Antibody (600-401-HB5) in IHC
Immunohistochemistry of Rabbit Anti-HEY1 Antibody. Tissue: Human Cerebellum. Fixative: none. Antigen Retrieval: HIER using Citrate buffer for 20 minutes. Primary Antibody: Anti-HEY1 at 1:200 for 30 minutes at RT. Secondary Antibody: Ready-to-Use Anti-Rabbit IgG HRP for 8 minutes at RT. Counterstain: Hematoxylin. Results: HEY1 showed diffuse cytoplasmic/membranous staining of low to moderate intensity of cells in the molecular layer, Pukinje cells layer, and granular cell layer in human cerebellum.
产品信息
600-401-HB5
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-HEY1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human HEY1 conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
1.06 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human HEY1. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse and 91.7% with dog and cattle.
靶标信息
Hairy/enhancer-of-split related with YRPW motif protein 1 belongs to the HEY family and is a Notch Signaling Pathway Protein. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. It is a downstream effector of Notch signaling which may be required for cardiovascular development. It acts as transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. It represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Hey1 is also reported to be expressed during development of the nervous system.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: basic helix-loop-helix protein OAF1; bHLHb31; Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split-related protein 1; Hairy-related transcription factor 1; Hairy/E(spl)-related with YRPW motif 1; hairy/enhancer-of-split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; hes-related family bHLH transcription factor with YRPW motif 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HRT-1; MGC1274; mHRT1
基因别名: AI316788; AI414254; BHLHB31; CHF2; HERP2; hesr-1; HESR1; HEY1; hHRT1; HRT-1; HRT1; OAF1
UniProt ID: (Human) Q9Y5J3
Entrez Gene ID: (Human) 23462, (Mouse) 15213
transcription factor activity, RNA polymerase II core promoter sequence-specific
transcription factor activity, protein binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
microsatellite binding
protein dimerization activity
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
sequence-specific DNA binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
pulmonary valve morphogenesis
atrioventricular valve formation
epithelial to mesenchymal transition involved in endocardial cushion formation
endocardial cushion morphogenesis
cardiac ventricle morphogenesis
transcription, DNA-templated
Notch signaling pathway
dorsal aorta morphogenesis
umbilical cord morphogenesis
negative regulation of Notch signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cardiac epithelial to mesenchymal transition
heart trabecula formation
cardiac septum morphogenesis
ventricular septum morphogenesis
labyrinthine layer blood vessel development
arterial endothelial cell differentiation
Notch signaling involved in heart development
cellular response to glucocorticoid stimulus
negative regulation of transcription regulatory region DNA binding
negative regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation
regulation of vasculogenesis
blood vessel development
vasculogenesis
osteoblast development
outflow tract morphogenesis
atrioventricular valve morphogenesis
endocardial cushion to mesenchymal transition involved in heart valve formation
ventricular trabecula myocardium morphogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
anterior/posterior axis specification
negative regulation of gene expression
mesenchymal cell development
negative regulation of neuron differentiation
positive regulation of osteoblast differentiation
artery development
