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HGAL Antibody (14-9758-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 5 µg/mL of Mouse IgG2a K Isotype Control Purified (left) or 5 µg/mL of Anti-Human HGAL Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
产品信息
14-9758-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
1H1-A7
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2572992
产品详细信息
Description: The monoclonal antibody 1H1-A7 recognizes human germinal center-associated lymphoma (HGAL) protein. HGAL is expressed in germinal centers, spleen, thymus, and germinal center-derived lymphomas. HGAL is localized to the cytoplasm of cells within the dark zone of the germinal center and functions to enhance the activation of RhoA leading to negative regulation of lymphocyte motility. HGAL expression has been used to help elucidate nodal marginal zone lymphoma (NMZL) from cases of diffuse follicle center lymphoma. Additionally, HGAL expression was shown to correlate with survival in patients with diffuse large B-cell lymphoma (DLBCL).
The 1H1-A7 antibody recognizes the protein product of the M17 gene, the mouse homolog of the HGAL protein.
Applications Reported: This 1H1-A7 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and ELISA.
Applications Tested: This 1H1-A7 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This 1H1-A7 antibody has been tested by western blot of reduced human and mouse lysate and can be used at less than or equal to 5 µg/mL. Iti s recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
GCET2 is a protein which may function in signal transduction pathways and whose expression is elevated in germinal cell lymphomas. It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4.This gene encodes a protein which may function in signal transduction pathways and whose expression is elevated in germinal cell lymphomas. It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4. Alternative splicing results in multiple transcript variants encoding different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: GCET2 germinal center expressed transcript 2; Germinal center associated lymphoma (hGAL); Germinal center B-cell-expressed transcript 2 protein; germinal center expressed transcript 2; Germinal center-associated lymphoma protein; Germinal center-associated signaling and motility protein; germinal center-associated, signaling and motility; hGAL; human germinal center-associated lymphoma; MGC40441
基因别名: GAL; GCAT2; Gcet; GCET2; GCSAM; HGAL; M17; M17-L
UniProt ID: (Human) Q8N6F7, (Mouse) Q6RFH4
Entrez Gene ID: (Human) 257144, (Mouse) 14525
