Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 6
HIF-2 alpha Antibody (MA5-27743) in ICC/IF
HIF-2 alpha Monoclonal Antibody (GT125) detects HIF2 alpha protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF2 alpha protein stained by HIF-2 alpha Monoclonal Antibody (GT125) (Product # MA5-27743) diluted at 1:100. Red: Actin filaments were labeled with Phalloidin.
Product Details
MA5-27743
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT125
Immunogen
Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735218
Product Specific Information
Positive Control: HepG2 (1% O2 treatment for 24hr)
Predicted Reactivity: Pig (83%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP2; bHLHe73; Class E basic helix-loop-helix protein 73; Endothelial PAS domain-containing protein 1; EPAS-1; Hif like protein; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2 alpha; HIF-2-alpha; HIF-related factor; HIF1 alpha-like factor; HIF1alpha-like factor; HIF2 alpha; HIF2-alpha; HLF; HLF (HIF1alpha-like factor); HRF; hypoxia inducible factor 2, alpha subunit; hypoxia inducible factor 2a; hypoxia inducible transcription factor 2alpha; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; mHLF; PAS domain-containing protein 2
Gene Aliases: BHLHE73; ECYT4; EPAS1; HIF-2alpha; HIF2A; HLF; HRF; MOP2; PASD2
UniProt ID: (Human) Q99814, (Mouse) P97481, (Rat) Q9JHS1
Entrez Gene ID: (Human) 2034, (Mouse) 13819, (Rat) 29452
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
protein binding
transcription factor binding
histone acetyltransferase binding
sequence-specific DNA binding
protein heterodimerization activity
transcription factor activity, sequence-specific DNA binding
signal transducer activity
protein dimerization activity
basic helix-loop-helix transcription factor
angiogenesis
response to hypoxia
embryonic placenta development
blood vessel remodeling
regulation of heart rate
transcription from RNA polymerase II promoter
mitochondrion organization
signal transduction
visual perception
erythrocyte differentiation
lung development
somatic stem cell population maintenance
norepinephrine metabolic process
surfactant homeostasis
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of transcription from RNA polymerase II promoter
cell maturation
myoblast fate commitment
iron ion homeostasis
regulation of transcription from RNA polymerase II promoter in response to hypoxia
cellular response to hypoxia
transcription, DNA-templated
regulation of transcription, DNA-templated
response to oxidative stress
multicellular organism development
hemopoiesis
cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support