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HIF1AN Antibody (PA5-34852) in ICC/IF
HIF1AN Polyclonal Antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN Polyclonal Antibody (Product # PA5-34852) diluted at 1:500. Blue: Hoechst 33342 staining.
产品信息
PA5-34852
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 78 and 349 of HIF1AN (Uniprot ID#Q9NWT6)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2552204
产品详细信息
Recommended positive controls: HCT116, MCF-7.
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (90%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH 1; FIH-1; FLJ20615; FLJ22027; hypoxia inducible factor 1, alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
基因别名: FIH1; HIF1AN
UniProt ID: (Human) Q9NWT6
Entrez Gene ID: (Human) 55662
Notch binding
iron ion binding
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxygen sensor activity
carboxylic acid binding
peptidyl-histidine dioxygenase activity
peptidyl-asparagine 3-dioxygenase activity
protein homodimerization activity
cofactor binding
NF-kappaB binding
ankyrin repeat binding
transcription, DNA-templated
peptidyl-histidine hydroxylation
peptidyl-aspartic acid hydroxylation
peptidyl-asparagine hydroxylation
positive regulation of myoblast differentiation
negative regulation of Notch signaling pathway
oxidation-reduction process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of vasculogenesis
