Invitrogen

HIPK2 Recombinant Rabbit Monoclonal Antibody (23GB2500)

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抗体检测数据 (2)

HIPK2 Antibody in Immunocytochemistry (ICC/IF)

HIPK2 Antibody in Immunocytochemistry (ICC/IF)

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HIPK2 Antibody (MA5-52506) in ICC/IF

Immunocytochemistry analysis of HIPK2 in HepG2 cells. Nuclei were stained blue with DAPI; HIPK2 recombinant monoclonal antibody (Product # MA5-52506) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

HIPK2 Antibody in Immunocytochemistry (ICC/IF)

HIPK2 Antibody in Western Blot (WB)

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HIPK2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of HIPK2 in HepG2 cells. Nuclei were stained blue with DAPI; HIPK2 recombinant monoclonal antibody (Product # MA5-52506) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

HIPK2 Antibody in Western Blot (WB) — Western Blot analysis of HIPK2. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HIPK2 recombinant monoclonal antibody (Product # MA5-52506) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

产品信息

MA5-52506

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

-

免疫细胞化学 (ICC/IF)

1:1,000

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB2500

抗原

A synthesized peptide derived from human HIPK2 (150-200AA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

HIPK2 is a nuclear serine/threonine kinase that is linked to transcriptional regulation and apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: hHIPk2; Homeodomain-interacting protein kinase 2

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基因别名: HIPK2; PRO0593

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UniProt ID: (Human) Q9H2X6

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Entrez Gene ID: (Human) 28996

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RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity transcription corepressor activity protein kinase activity protein serine/threonine kinase activity protein binding ATP binding SMAD binding virion binding

negative regulation of transcription from RNA polymerase II promoter eye development transcription, DNA-templated protein phosphorylation DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator transforming growth factor beta receptor signaling pathway smoothened signaling pathway adult walking behavior positive regulation of cell proliferation anterior/posterior pattern specification retina layer formation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation modulation by virus of host morphology or physiology neuron differentiation erythrocyte differentiation positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway PML body organization positive regulation of protein binding intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of DNA binding negative regulation of neuron apoptotic process positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade embryonic camera-type eye morphogenesis voluntary musculoskeletal movement positive regulation of sequence-specific DNA binding transcription factor activity regulation of cell cycle embryonic retina morphogenesis in camera-type eye lens induction in camera-type eye SMAD protein signal transduction iris morphogenesis cellular response to hypoxia intrinsic apoptotic signaling pathway regulation of signal transduction by p53 class mediator