Invitrogen

HIRA Polyclonal Antibody

HIRA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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HIRA Antibody (PA5-97065) in IHC (P)

Immunohistochemistry analysis of HIRA in paraffin-embedded human colon carcinoma. Samples were incubated with HIRA Polyclonal antibody (Product # PA5-97065) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Product Image Gallery

HIRA Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot of HIRA using anti-HIRA polyclonal antibody (Product # PA5-97065) shows decrease in expression of HIRA upon treatment with Doxycycline. {TM}

产品信息

PA5-97065

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_0033163)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2808867

产品详细信息

Immunogen sequence: AQIIEREGWK TNMDFVGHRK AVTVVKFNPK IFKKKQKNGS SAKPSCPYCC CAVGSKDRSL SVWLTCLKRP LVVIHELFDK SIMDISWTLN GLGILVCSMD GSVAFLDFSQ DELGDPLSEE EKSRIHQSTY GKSLAIMTEA QLSTAVIENP EMLKYQRRQQ QQQLDQKSAA TREMGSATSV AGVVNGESLE DIRKNLLKKQ VETRTADGRR RITPLCIAQL DTGDFSTAFF NSIPLSGSLA GTMLSSHSSP QLLPLDSSTP NSFGASKPCT EPVVAASARP AGDSVNKDSM NATSTPAALS P; Positive Samples: 293T, HepG2, HeLa; Cellular Location: Nucleus, PML body

靶标信息

HIRA is a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this protein in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochromatin. It plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate protein in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。