Invitrogen

HK2 Monoclonal Antibody (3D3)

HK2 Antibody in Immunocytochemistry (ICC/IF)

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HK2 Antibody (MA5-15679) in ICC/IF

Immunofluorescence analysis of HK2 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HK2 Monoclonal Antibody (3D3) (Product # MA5-15679) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More

HK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HK2 Antibody in Immunohistochemistry (IHC)

HK2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

HK2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inHK2 KO cell line compared to control cell line using HK2 Monoclonal Antibody (3D3) (Product # MA5-15679). {KO}

产品信息

MA5-15679

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:100

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流式细胞分析 (Flow)

1:200-1:400

酶联免疫吸附实验 (ELISA)

1:10,000

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3D3

抗原

Purified recombinant fragment of human HK2 expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

ascites

内含物

0.03% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_10986812

产品详细信息

MA5-15679 targets HK2 in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples.

The MA5-15679 immunogen is purified recombinant fragment of human HK2 expressed in E. Coli.

MA5-15679 detects HK2 which has a predicted molecular weight of approximately 102kDa.

靶标信息

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hypoxia induces a glycolytic complex in intestinal epithelial cells independent of HIF-1-driven glycolytic gene expression.

Proceedings of the National Academy of Sciences of the United States of America

Kierans SJ,Fagundes RR,Malkov MI,Sparkes R,Dillon ET,Smolenski A,Faber KN,Taylor CT

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Western Blot

Tue Aug 29 00:00:00 UTC 2023

Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia.

Journal of bone oncology

Yang J,Liu Z,Liu B,Sun L

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunohistochemistry

Thu Dec 01 00:00:00 UTC 2022

GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.

International journal of molecular sciences

Marwarha G,Røsand Ø,Slagsvold KH,Høydal MA

MA5-15679 was used in Enzyme-linked immunosorbent assay to unveil that the inhibition of GSK3β kinase activity is indispensable in mediating the miR-210-orchestrated protective cellular response to hypoxia-induced apoptotic cell death.

Fri Aug 19 00:00:00 UTC 2022

Higher Glucose Enhances Breast Cancer Cell Aggressiveness.

Nutrition and cancer

Santos JM,Hussain F

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Western Blot

Wed Apr 14 00:00:00 UTC 2021

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

Targeting metabolic plasticity in glioma stem cells in vitro and in vivo through specific inhibition of c-Src by TAT-Cx43_266-283.

EBioMedicine

Pelaz SG,Jaraíz-Rodríguez M,Álvarez-Vázquez A,Talaverón R,García-Vicente L,Flores-Hernández R,Gómez de Cedrón M,Tabernero M,Ramírez de Molina A,Lillo C,Medina JM,Tabernero A

Published figure using HK2 monoclonal antibody (Product # MA5-15679) in Immunocytochemistry

Tue Dec 01 00:00:00 UTC 2020

生物信息学

蛋白别名: DKFZp686M1669; Hexokinase; Hexokinase type II; Hexokinase-2; hexokinase-2 muscle; hexokinase-2, muscle; Hexokinase-B; HK II; Muscle form hexokinase; OTTHUMP00000202738

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基因别名: HK2; HKII; HXK2

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UniProt ID: (Human) P52789

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Entrez Gene ID: (Human) 3099

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功能

glucokinase activity hexokinase activity protein binding ATP binding glucose binding fructokinase activity mannokinase activity

参与通路