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HK2 Antibody (MA5-14849) in WB
Knockout of HK2 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR1034965_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of HK2 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa HK2 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlo... View More
产品信息
MA5-14849
产品规格
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
H.738.7
抗原
Synthetic peptide corresponding to residues surrounding Ser340 of sequence of human hexokinase II.
偶联物
形式
Liquid
浓度
24 µg/mL
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10987229
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
