Invitrogen

HLA-BC Monoclonal Antibody (B1.23.2), APC, eBioscience™

HLA-BC Antibody in Flow Cytometry (Flow)

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HLA-BC Antibody (17-5935-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (blue histogram) or Anti-Human HLA-BC APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-5935-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

B1.23.2

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11151509

产品详细信息

Description: This B1.23.2 monoclonal antibody recognizes the human Human Leukocyte Antigens (HLA) B and C. Along with HLA-A, these proteins correspond to MHC Class I in humans. HLA-B and HLA-C are expressed on nearly all cell types and heterodimerize with beta-2 microglobulin at the cell surface. These molecular complexes play a critical role in the presentation of endoplasmic reticulum-derived peptides to immune cells.

Applications Reported: This B1.23.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B1.23.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Genome editing HLA alleles for a pilot immunocompatible hESC line in a Chinese hESC bank for cell therapies.

Cell proliferation

Ji TT,Niu SS,Fang MH,Xu LX,Wang X,Zou J,Xu F,Zhang M,Niu R,Wu J,Wang L,Li ZK,Yang YG,Hao J,Li W,Hu Z,Zhou Q

17-5935-42 was used in Flow Cytometry to explore the possibility of customizing immunocompatible hESCs based on Chinese HLA typing characteristics.

Mon May 01 00:00:00 UTC 2023

The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses.

Immunity

Jongsma MLM,de Waard AA,Raaben M,Zhang T,Cabukusta B,Platzer R,Blomen VA,Xagara A,Verkerk T,Bliss S,Kong X,Gerke C,Janssen L,Stickel E,Holst S,Plomp R,Mulder A,Ferrone S,Claas FHJ,Heemskerk MHM,Griffioen M,Halenius A,Overkleeft H,Huppa JB,Wuhrer M,Brummelkamp TR,Neefjes J,Spaapen RM

17-5935-42 was used in Flow Cytometry to identify a GSL signature that inhibits immune recognition and represents a potential therapeutic target in cancer, infection, and autoimmunity.

Tue Jan 12 00:00:00 UTC 2021

生物信息学

蛋白别名: class I MHC, HLA-B antigen; epididymis secretory protein Li 83; histocompatibility antigen; HLA -B; HLA B-40011; HLA B44; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, B-47 alpha chain; HLA class I histocompatibility antigen, B-50 alpha chain; HLA class I histocompatibility antigen, B-82 alpha chain; HLA locus; HLA molecule; HLA-B; HLA-B alpha chain; HLA-B alpha chain (B*2706); HLA-B alpha chain (B*5002); HLA-B alpha-chain; HLA-B35; HLA-B35 antigen; HLA-Bw50 antigen; Human leukocyte antigen B; hypothetical protein; lecocyte antigen B; Leukocyte Antigen; leukocyte antigen class I-B; Leukoocyte Antigen; lymphocyte antigen; major histocompatability complex B antigen; MHC antigen; MHC class 1 antigen; MHC class I alpha chain; MHC class I anitgen; MHC class I antigen B*47; MHC class I antigen B*50; MHC class I antigen B*82; MHC class I antigen GN00104; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I antigene; MHC class I human leukocyte antigen; MHC class I molecule; MHC class I protein; MHC class II antigen; MHC class l antigen; MHC classI antigen; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B27-HS; MHC HLA-B27d; MHC HLA-B39N; truncated class I antigen; truncated MHC class I antigen

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基因别名: AS; B-4901; B-5001; Bw-47; Bw-50; HEL-S-83; HLA-B; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HLA-Cw; HLA-DRB1; HLAB; SPDA1

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UniProt ID: (Human) Q31603

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Entrez Gene ID: (Human) 3106

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功能

antigen binding peptide antigen binding major histocompatibility complex protein

参与通路

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent viral process antigen processing and presentation regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway