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产品信息
11-9881-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
SK10
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10853328
产品详细信息
Description: This SK10 monoclonal antibody reacts with the human MHC Class II HLA-DQ molecule. This cell surface receptor is a heterodimeric protein composed of an alpha (HLA-DQA1) and beta (HLA-DQB1) subunit. HLA-DQ is expressed on antigen presenting cells such as B cells, monocytes, macrophages, dendritic cells, and activated T cells. HLA-DQ plays a role in presenting peptide antigens to CD4+ T lymphocytes. Reports indicate that certain alleles of HLA-DQ are associated with celiac disease and type I diabetes.
Applications Reported: This SK10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SK10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
HLA-DQ is one of the HLA (human leukocyte antigen) molecules belonging to class II of the major histocompatibility protein (MHC) surface proteins. HLA-DQ and the other MHC class II molecules are heterodimeric proteins consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. They play a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages) and present peptides endocytosed, digested, and loaded onto the proteins prior to their migration to the cell surface. Other human MHC class II molecules are HLA-DP, HLA-DM, HLA-DR, HLA-DOA, and HLA-DOB. HLA-DQ displays significant variability, particularly in the beta subunit within the region that affects the peptides bound and presented by the protein. Different classes of DQ based on similar beta subunits allow for the grouping of these variants into serotypes, which can often be recognized by antibodies raised against these subunits.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: HLA class II antigen; HLA class II histocompatibility antigen, DQ beta 1 chain; lymphocyte antigen; MHC class II antigen DQB1; MHC class II antigen HLA-DQ-beta-1; MHC class II DQ beta chain; MHC class II HLA-DQ beta glycoprotein; MHC class2 antigen; MHC DQ beta
基因别名: CELIAC1; HLA-DQB; HLA-DQB1; HLA-DRB1; IDDM1
UniProt ID: (Human) C9DRQ3
Entrez Gene ID: (Human) 3119
immunoglobulin production involved in immunoglobulin mediated immune response
humoral immune response mediated by circulating immunoglobulin
immune response
antigen processing and presentation of exogenous peptide antigen via MHC class II
T cell costimulation
T cell receptor signaling pathway
interferon-gamma-mediated signaling pathway
