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FIGURE: 1 / 1
HLA-F Antibody (PA5-87858) in WB
Western blot analysis of HLA-F in extracts of A-549 cells. Samples were incubated with HLA-F Polyclonal antibody (Product # PA5-87858) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-87858
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human HLA-F (NP_0010919491)
Conjugate
Form
Liquid
Concentration
1.93 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804461
Product Specific Information
Immunogen sequence: GSHSLRYFST AVSRPGRGEP RYIAVEYVDD TQFLRFDSDA AIPRMEPREP WVEQEGPQYW EWTTGYAKAN AQTDRVALRN LLRRYNQSEA GSHTLQGMNG CDMGPDGRLL RGYHQHAYDG KDYISLNEDL RSWTAADTVA QITQRFYEAE EYAEEFRTYL EGECLELLR; Positive Samples: A-549; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDA12; HLA class I histocompatibility antigen, alpha chain F; HLA class I molecule; HLA F antigen; Leukocyte antigen F; MHC class I antigen F; MHC class Ib antigen
Gene Aliases: CDA12; HLA-5.4; HLA-CDA12; HLA-F; HLAF
UniProt ID: (Human) P30511
Entrez Gene ID: (Human) 3134
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent
regulation of immune response
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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