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HMG4 Antibody (MA5-36230) in ICC/IF
Immunocytochemistry analysis of HMG4 in HeLA cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HMG4 monoclonal antibody (Product # MA5-36230) at a dilution of 2 µg/mL (overnight, 4°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C).
产品信息
MA5-36230
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
7G13
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4 (62-95aa EMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKR).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2884064
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
HMGB3 belongs to the high mobility group protein superfamily. Like HMG1 and HMG2, HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; high-mobility group (nonhistone chromosomal) protein 4; HMG-2a; HMG-4; MGC90319
基因别名: HMG-2a; HMG-4; HMG2A; HMG4; HMGB3
UniProt ID: (Human) O15347
Entrez Gene ID: (Human) 3149
