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Invitrogen
HMGA2 Polyclonal Antibody
FIGURE: 1 / 13
HMGA2 Antibody (PA5-21320) in ICC/IF
HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody (Product # PA5-21320) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Product Details
PA5-21320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 109 of HMGA2 (Uniprot ID#P52926)
Conjugate
Form
Liquid
Concentration
0.08 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Dry ice
RRID
AB_11155799
Product Specific Information
Recommended positive controls: A431, HeLa, HepG2, HCT116.
Predicted reactivity: Mouse (95%), Rat (92%), Zebrafish (85%), Xenopus laevis (80%), Chicken (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 shares 95% amino acid sequence identity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: High mobility group AT-hook protein 2; High mobility group protein HMGI-C; high mobility group protein I, isoform C; high-mobility group (nonhistone chromosomal) protein isoform I-C; High-mobility group protein HMGI-C; non-histone chromosomal architectural protein HMGI-C
Gene Aliases: 9430083A20Rik; BABL; HMGA2; HMGI-C; HMGIC; LIPO; pg; pygmy; STQTL9
UniProt ID: (Human) P52926, (Mouse) P52927
Entrez Gene ID: (Human) 8091, (Mouse) 15364, (Rat) 84017
regulatory region DNA binding
transcription factor activity, transcription factor binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
AT DNA binding
DNA-(apurinic or apyrimidinic site) lyase activity
DNA-dependent protein kinase activity
protein binding
transcription factor binding
DNA binding, bending
nucleosomal DNA binding
cAMP response element binding
MH2 domain binding
MH1 domain binding
SMAD binding
5'-deoxyribose-5-phosphate lyase activity
C2H2 zinc finger domain binding
enzyme binding
transcription factor activity, sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
base-excision repair
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle
mitotic nuclear division
signal transduction
spermatogenesis
cell proliferation
positive regulation of cell proliferation
male gonad development
regulation of cell cycle process
positive regulation of gene expression
cell proliferation in forebrain
pituitary gland development
chromosome condensation
adrenal gland development
negative regulation of intracellular steroid hormone receptor signaling pathway
somatic stem cell population maintenance
regulation of growth
positive regulation of multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
negative regulation of JAK-STAT cascade
negative regulation of astrocyte differentiation
positive regulation of sequence-specific DNA binding transcription factor activity
cell division
meiotic cell cycle
regulation of growth hormone secretion
adipose tissue development
fat pad development
regulation of peptide hormone secretion
nucleic acid phosphodiester bond hydrolysis
positive regulation of stem cell proliferation
epithelial to mesenchymal transition
chondrocyte differentiation
mesodermal-endodermal cell signaling
chromatin organization
mitotic G2 DNA damage checkpoint
multicellular organism development
response to virus
chromosome breakage
heterochromatin assembly
histone H2A-S139 phosphorylation
senescence-associated heterochromatin focus assembly
endodermal cell differentiation
chondrocyte proliferation
DNA damage response, detection of DNA damage
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of DNA binding
negative regulation by host of viral transcription
fat cell differentiation
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
mesodermal cell differentiation
mesenchymal cell differentiation
stem cell differentiation
positive regulation of cell cycle arrest
oncogene-induced cell senescence
regulation of stem cell population maintenance
positive regulation of transcription regulatory region DNA binding
positive regulation of cellular response to X-ray
positive regulation of cellular senescence
positive regulation of response to DNA damage stimulus
negative regulation of double-strand break repair via nonhomologous end joining
regulation of cellular response to drug
DNA catabolic process, endonucleolytic
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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