Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
HMGB2 Antibody (PA5-120546) in WB
Western blot analysis of HMGB2 in extracts of various cell lines. Samples were incubated with HMGB2 Polyclonal antibody (Product # PA5-120546) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-120546
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB2 (NP_002120.1).
偶联物
形式
Liquid
浓度
2.44 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914118
产品详细信息
Positive test controls include: HeLa, 293T, U-87MG, RAW264.7, PC-12. The target is usually found in the following locations: Chromosome, Cytoplasm, Nucleus, Secreted.
Immunogen sequence: SAFFLFCSEH RPKIKSEHPG LSIGDTAKKL GEMWSEQSAK DKQPYEQKAA KLKEKYEKDI AAYRAKGKSE AGKKGPGRPT GSKKKNEPED EEEEEEEEDE D
靶标信息
HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: High mobility group protein 2; High mobility group protein B2; high-mobility group (nonhistone chromosomal) protein 2; high-mobility group box 2; HMG 2; HMG B2; HMG-2; HMG-B2; HMGB-2
基因别名: C80539; HMG-2; HMG2; HMGB2
UniProt ID: (Human) P26583, (Rat) P52925, (Mouse) P30681
Entrez Gene ID: (Human) 3148, (Rat) 29395, (Mouse) 97165
four-way junction DNA binding
enhancer sequence-specific DNA binding
DNA binding
damaged DNA binding
double-stranded DNA binding
single-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
drug binding
DNA binding, bending
protein domain specific binding
chemoattractant activity
transcription regulatory region DNA binding
non-sequence-specific DNA binding, bending
poly(A) RNA binding
RAGE receptor binding
supercoiled DNA binding
lipid binding
nucleosomal histone binding
chromatin binding
positive regulation of endothelial cell proliferation
base-excision repair, DNA ligation
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
spermatogenesis
spermatid nucleus differentiation
nervous system development
male gonad development
organ regeneration
positive regulation of nuclease activity
positive regulation of DNA binding
positive regulation of erythrocyte differentiation
positive regulation of megakaryocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to steroid hormone
positive chemotaxis
positive regulation of DNA metabolic process
cell chemotaxis
cellular response to lipopolysaccharide
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
inflammatory response to antigenic stimulus
DNA topological change
apoptotic DNA fragmentation
chromatin organization
nucleosome assembly
transcription, DNA-templated
DNA geometric change
response to lipopolysaccharide
positive regulation of interferon-beta production
V(D)J recombination
response to drug
innate immune response
positive regulation of innate immune response
regulation of neurogenesis
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
DNA ligation involved in DNA repair
regulation of stem cell proliferation
